|  BV-2 cells are semi-adherent cells and I've noticed that my BV-2 cells fall down really easily. 93112520). Transcriptome (480 genes) and proteome analyses after stimulation with lipopolysaccharide indicated a reaction pattern of BV-2 with many similarities to that of PM, although the average upregulation of genes was less pronounced. BV-2 cells are semi-adherent cells and I've noticed that my BV-2 cells fall down really easily. Established in 1925 to serve as a national center for depositing and distributing microbiological specimens, ATCC has since grown to distribute in over 150 countries. Epub 2019 Feb 13. Current affiliation: CB: Emory University, Atlanta, GA; GC: University of Alabama at Birmingham, Birmingham, AL; CP: Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USA. Epub 2016 May 9. Human cell lines, mouse cell lines, cancer cell lines and more are available. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/murine microglial cell line bv2/product/ATCC ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/murine microglia cell line bv2/product/ATCC Average 92 stars, based on 3 article reviews Bulk and Prepack available at Sigmaaldrich.com. Special Features of the Cell Line Viruses: SMRV: Negative, as confirmed by Real-Time PCR . Attached cells may be subcultured by tapping the sides of the flask until cells are dispersed. In view of the fact that "being now authenticated by ATCC®" may imply a wider distribution of the cells, we aimed at reviewing data obtained with the human microglia cell line clone 3, making the readers aware of this complicated nomenclature. One thousand cells/well were counted, and bars represent the standard deviations of results of three separate experiments performed in … B. Nitrite accumulation in BV-2 cells following 18 h exposure to FRs at 10 μM or 40 μM for 18 h … ATCC CRL-1772 myotube formation. 2020 Nov 3;17(1):327. doi: 10.1186/s12974-020-01999-8. ATCC was entrusted with its first cell line in 1962 and has consistently attained the highest standards and used the most reliable procedures to verify every cell line since. The protein samples of each fraction were treated the same way as the SH-SY5Y protein samples. ECACC primary cell lines are offered from a variety of mammalian sources. 2020 Sep 11;11:1315. doi: 10.3389/fphar.2020.01315. The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 2007 Aug;7(8):1092-101. doi: 10.1016/j.intimp.2007.04.005. CHO cell lines: GS system, DHFR system; BHK cell line; Mouse myeloma cell lines: NS0, SP2/0, etc. It is also one of the oldest; it was established in 1990, through retroviral transduction with the v-myc and v-raf oncogenes. With a wealth of cell-based immortalization experience, AcceGen scientists have been able to successfully immortalize a variety of mammalian cells … So is it necessary to use 0.25% trypsin to digest the cells in subculture? However, exposure related issues for susceptible populations, the developing, infirmed, or aged, remain given environmental persistence and home-environment detection. Immortalization of B lymphocytes by EBV is an effective procedure for inducing long-term growth of certain human B lymphocytes. The microglial cell line, BV-2, is cultured every 3-4 days in new medium in a T75 flask. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.  |  Cells grow as a monolayer and in suspension in spinner cultures. By continuing you agree to the use of cookies. A human myeloblastic cell line, MDS-L 20 was derived as a subline from a myelodysplastic cell line, MDS92 which was established from the bone marrow of … Kim HW, Kim J, Kim J, Lee S, Choi BR, Han JS, Lee KW, Lee HJ. Click to retrieve the detailed description of the cell line, for a representative picture of the cell line in culture, here for displaying the abbreviations used in the description. BV-2 cells [American Type Culture Collection (ATCC), Wesel, Germany] at <10 passages were cultured. With the use of the T75 flask I am able to recover about 10-15 million cells after 3-4 days from just 1-2 million cells originally. Use of a microglia-like cell line would accelerate many research programmes and reduce the necessity of continuous cell preparations and animal experimentation, provided that the cell line reproduces the in vivo situation or primary microglia (PM) with high fidelity. The A2780 human ovarian cancer cell line was established from tumour tissue from an untreated patient. NIH The mouse microglial cell line BV-2 (ATCC, Rockville, MD, USA) was routinely cultured in Roswell Park Memorial Institute (RPMI) 1640 medium, supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, 100 mg/mL streptomycin and 2 mM l-glutamine at 37 °C in a humidified 5%-CO 2 incubator. Usually BV-2 cells grow every 2 days (and fill up a 75cm2 flask) so you need to subculture. 2020 Dec;16(12):e9819. J Neuroinflammation. As representative of the nervous system innate immune system, the current study examined changes in microglia following exposure to representative FRs, pentabromophenol (PBP), tetrabromobisphenol A (2,2′,6,6′,-tetrabromo-4,4′-isopropylidine diphenol; TBBPA) and triphenyl phosphate (TPP). Cells were cloned in soft agar in the presence of CSF-1 and expanded on microcarrier beads. Cell Culture and Co-Culture Experiments Mouse microglia cell line BV2 and mouse hippocampal neuronal cell line HT22 (ATCC, Manassas, VA) were cultured in DMEM containing 10% FBS and 1% penicillin–streptomycin solution, and incubated at 37 °C in 5% CO2 . The protocol described in this unit to accomplish this can be divided into three stages: preparation of virus, preparation of target cells to be immortalized, and EBV infec … Mineralocorticoid and glucocorticoid receptors differentially regulate NF-kappaB activity and pro-inflammatory cytokine production in murine BV-2 microglial cells. Morshed N, Ralvenius WT, Nott A, Watson LA, Rodriguez FH, Akay LA, Joughin BA, Pao PC, Penney J, LaRocque L, Mastroeni D, Tsai LH, White FM. ScienceDirect ® is a registered trademark of Elsevier B.V. ScienceDirect ® is a registered trademark of Elsevier B.V. Mitochondrial-related effects of pentabromophenol, tetrabromobisphenol A, and triphenyl phosphate on murine BV-2 microglia cells, https://doi.org/10.1016/j.chemosphere.2020.126919. Moon DO, Park SY, Lee KJ, Heo MS, Kim KC, Kim MO, Lee JD, Choi YH, Kim GY. It is very important that these cells be cultured in the ATCC-recommended medium and seeded at a … ECACC primary cell lines are offered from a variety of mammalian sources. In this regard, reports of flame retardant (FR)-related effects on the innate immune system suggest process by which a spectrum of adverse health effects could manifest across the life-span. BV-2 were also able to stimulate other glial cells. Bee venom and melittin reduce proinflammatory mediators in lipopolysaccharide-stimulated BV2 microglia. Popular TaqMan Real-Time PCR Assays Antibodies Oligos, Primers & Probes GeneArt Gene Synthesis Cell Culture Plastics; Applications & Techniques ATCC murine microglia cell line bv2 Murine Microglia Cell Line Bv2, supplied by ATCC, used in various techniques. Primary cells Choose from our extensive catalog of primary cells from normal and diseased lung tissue. Values of nitrite accumulation from treated cells represent the mean ± SEM of two independent experiments conducted in tetraplicates. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. eCollection 2020. Dong L, Li YZ, An HT, Wang YL, Chen SH, Qian YJ, Wang K, Zhen JL, Fan Z, Gong XL, Zheng Y, Wang XM. PBP, showed a direct stimulation of aggregate formation and properties as a NLRP3 inflammasome secondary trigger. Cells grow as a monolayer and in suspension in spinner cultures. Murine cultured microglial cells were immortalized after infection with a v‐raf/v‐myc recombinant retro‐virus. About 95% of these cells were positive for CD11b (cluster of differentiation molecule 11B), a marker for microglial cell types. BC-2 is a lymphoma cell line originated in 1992 by Ethel Cesarman and Giorgio Inghirami in the laboratory of Daniel M. Knowles. Here, we re-evaluated strengths and potential short-comings of BV-2 cells. PBP and TBBPA diminished phagocytic capability. This site needs JavaScript to work properly. .. HT22 cells were starved before stimulation for 8 h and then stimulated with macrophage colony-stimulating factor (M-CSF) (40 ng/ml, … For non-commercial investigative use only. The immortalised murine microglial cell line BV-2 has been used frequently as a substitute for PM, but recently doubts were raised as to their suitability. *Please select more than one item to compare The in vitro calcification of primary mouse, human and bovine vascular smooth muscle cells (VSMCs) is commonly employed to examine the mechanisms of … BV-2 cells are derived from primary mouse microglia cells (Blasi, Barluzzi, Mazzolla, & Bistoni, 1990). This immortalized cell line (BV‐2) shares properties with body macrophages with respect to the antigen profile, their phagocytic capacity and antimicrobial activity. Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. A2780 Cell Line; The A2780 human ovarian cancer cell line was established from tumor tissue from an untreated patient. NLM Cell culture guidelines The following is a general guideline for culturing of cell lines. Indole-3-carbinol regulates microglia homeostasis and protects the retina from degeneration. PBP and TBBPA showed potential as trigger for NLRP3 inflammasome activatio. BV-2 cells are semi-adherent cells and I've noticed that my BV-2 cells fall down really easily. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. The cells were derived from a pleural cavity based lymphomatous effusion. Anti-Inflammatory Property of the Essential Oil from. Cell Line List Click to retrieve the detailed description of the cell line ... BV-2: mouse microglial cells, recombinant retrovirus (v-raf/v-mic) transformed: ATL03001: BxPC-3: human pancreas adenocarcinoma: HTL96011: C2-Rev7: rat hepatoma: ATL96007: C33A: human cervix carcinoma: HTL02004: C6: Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Phagocytosis was decreased for PBP and TBBPA. 2010 Mar 31;1322:1-7. doi: 10.1016/j.brainres.2010.01.082. SIM-A9 cells expressing microglia/macrophage-specific proteins. ZERO BIAS - scores, article reviews, protocol conditions and more Mol Syst Biol. Cell line available for distribution. ATCC murine microglial cell line bv2 Murine Microglial Cell Line Bv2, supplied by ATCC, used in various techniques. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. ATCC bv2 cells Bv2 Cells, supplied by ATCC, used in various techniques. PROPERTIES: The BV-2 cells express the nuclear v-myc and the cytoplasmic v-raf oncogene products as well as the env gp70 antigen at the surface level; the BV-2 cells have morphological, … ATCC or the American Type Culture Collection is a nonprofit organization which collects, stores, and distributes standard reference microorganisms, cell lines and other materials for research and development. BV-2 cells are a useful cell line because they can be maintained in culture, yet they keep many of the functions and features that microglia express in vivo (Henn, Lund, Hedtjarn, Schrattenholz, Porzgen, & … The BV-2 cells express the nuclear v-myc and the cytoplasmic v-raf oncogene products as well as the env gp70 antigen at the surface level; The BV-2 cells have morphological, phenotypical … TBBPA showed indications of possible secondary triggering activity while no changes were seen with TPP. 2012 Nov 28;9:260. doi: 10.1186/1742-2094-9-260. RESULTS: DHA (12.5-100 μM), 4-HHE (1.25-10 μM), and 4-HNE (1.25-10 μM) dose dependently suppressed LPS-induced production of NO, ROS, and as p-cPLA2 in BV-2 microglial cells. J. Neurochem. Popular. Please enable it to take advantage of the complete set of features! CNS Neurosci Ther. This is an immortalized cell line derived from the brain of an apparently normal 10 day old mouse. Alternatively I might use the human cell line HMC3, available from ATCC. Search results for bv2 cell at Sigma-Aldrich. BV‐2 cells are not constitu‐tively able to kill tumor cells in vitro, but acquire antitumor activity following an increase in [Ca ++] i. BV‐2 cells, like microglial cells, are however, distinct from peripheral macrophages by their expression of inwardly rectifying K + cannels in concert with a Jack in outwardly rectifying K + channels and the formation of spineous processes. Sigma-Aldrich has partnered with The European Collection of Cell Cultures, ECACC, a world leader and recognized expert in the maintenance, cultivation and distribution of authenticated cell lines. HHS Custom Cell Immortalization Service AcceGen has developed the proprietary Cell Immortalization Service for research application. Cellosaurus - a cell line knowledge resource, NCI CPTC Antibody Characterization Program. To make the complete growth medium, add the following components to the base medium: additional 2 mM L-glutamine; additional 1mM sodium pyruvate; adjust to a final concentration of 10% fetal bovine serum and 10% T-STIM with Con A. T-STIM is available from Becton Dickinson. Remarkably, the same cell line has been used in different laboratories with other denominations, i.e., CHME-5 cells and C13-NJ cells. COVID-19 is an emerging, rapidly evolving situation. Beads were transferred to culture dishes and were subsequently passaged by scraping. A2780 is the parent line to the cisplatin resistant cell line A2780 cis (Sigma catalogue no. Thus, BV-2 cells appear to be a valid substitute for PM in many experimental settings, incuding complex cell-cell interaction studies. BV-2 cells were fractionated immediately after collection using Subcellular Protein Fractionation Kit for Cultured Cells (Thermo Fisher Scientific Inc.). A. Maintain cell density between 1 x 10 5 and 1 x 10 6 viable cells/mL. 1. The ATCC Cell Biology Collection is one of the largest bioresources in the world, and offers a complex array of human, animal, insect, fish and stem cell lines from which to choose. 2016 Aug;22(8):661-9. doi: 10.1111/cns.12557. Following 18hr exposure of murine BV-2 cells, at dose levels resulting in ≥80% viability (10 and 40 μM), limited alterations in pro-inflammatory responses were observed however, changes were observed in mitochondrial respiration. ATCC offers a broad portfolio of primary and immune cell lines needed to support research and aid in the development of SARS-CoV-2 antivirals, vaccines, and therapeutics. The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, (ATCC ® 30-2001™). Cultures can be established between 1 x 10 4 and 4 x 10 4 viable cells/cm 2. Microglia cell line BV-2 purchased from ATCC was cultured in DMEM medium with 10% FBS, 100 U/ml penicillin and 100 µg/ml streptomycin at 37°C with 5% CO 2. ATCC Quality Control Methods for Cell Lines, 2nd edition, 1992. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). 2014 Jan;137(1):158-67. doi: 10.1093/toxsci/kft240. Subculturing Cultures can be maintained by the addition of fresh medium or replacement of medium. ATCC was entrusted with its first cell line in 1962 and has consistently attained the highest standards and used the most reliable procedures to verify every cell line since. Transcriptome sequencing reveals that LPS-triggered transcriptional responses in established microglia BV2 cell lines are poorly representative of primary microglia. Cell culture guidelines The following is a general guideline for culturing of cell lines. J Neuroinflammation. Murine Microglial Cell Lines. 4.2. Thus, the data suggests an effect of all three FRs on mitochondria metabolism yet, different functional outcomes including, phagocytic capability and NLRP3 inflammasome activation. So is it necessary to use 0.25% trypsin to digest the cells in subculture? So is it necessary to use 0.25% trypsin to digest the cells in subculture? BV-2 cells are semi-adherent cells and I've noticed that my BV-2 cells fall down really easily. PBP, TBBPA, or TPP exposure modified mitochondrial bioenergetics of microglia. The C2C12 murine myoblast cell line (CRL-1772; ATCC) was cultured in low glucose DMEM (Wisent) supplemented with 10% FBS, 100IU/mL penicillin and 100mg/mL streptomycin. If you fail to find your cell line of interest, it is suggested to identify similar cell lines that may act as a good starting point for your own research. Phosphoproteomics identifies microglial Siglec-F inflammatory response during neurodegeneration. Preparing an aseptic environment 1. Tissue: brain, microglial cells; Transformed by: recombinant retrovirus (v-raf/v-mic). SIM-A9 cells were treated without (Control; left panels) or with 2.5 ng/mL LPS(LPS; right panels) for 24 h prior to immunostaining for microglial markers, CD68 or Iba1, as well as for astrocyte and … Generation of conditioned Medium LADMAC conditioned medium is made from LADMAC cells (ATCC CRL-2420). Cell viability of BV-2 cells following 18 h post-exposure to PBP, TBBPA, or TPP at various concentrations. CRISPR/Cas9-mediated knockout of Abcd1 and Abcd2 genes in BV-2 cells: novel microglial models for X-linked Adrenoleukodystrophy Biochim Biophys Acta Mol Cell Biol Lipids. Clipboard, Search History, and several other advanced features are temporarily unavailable. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). The BV-2 cell line, developed by Blasi and colleagues [9], is one of the most popular microglial cell lines ever created. BV- 2 细胞系为Blasi 等 在1990 年应用携带癌基因v- raf/ v- myc 的反转录病毒J2 感染原代培养的小鼠小胶质细胞而获得的永生细胞系， 该细胞系不仅高度纯化， 而且基本具备了原代培养的小胶质细胞的形态学、表型以及各项功能特点， 相对较易培养， 被国外许多学者所应用。 A2780 Cell Line; Recommended culture medium: RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS). Human cell lines: HEK293 and its derivatives, HT-1080, Huh-7, PER.C6, etc. Wechat. HeLa S3 cells, derived from a human cervical epithelial cell line (CCl-2.2; ATCC), were cultured in the same manner as BV-2 and RAW264.7 cell lines. 30-2002. Chen J, Tang C, Zhou Y, Zhang R, Ye S, Zhao Z, Lin L, Yang D. Molecules. Add appropriate aliquots of the cell suspension to new culture vessels. Their response to lipopolysaccharide was compared with the response of microglia in vitro and in vivo. Epub 2013 Oct 26. Epub 2007 May 2. Compare Products: Select up to 4 products. . BC-2 is a lymphoma cell line originated in 1992 by Ethel Cesarman and Giorgio Inghirami in the laboratory of Daniel M. Knowles. With the same concentrations, these compounds could enhance Nrf2 and HO-1 expression in these cells. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density). doi: 10.15252/msb.20209819. BV-2 (mouse, C57BL/6, brain, microglial cells)ICLC catalog code: ATL03001 Continuous culture, grown as semiadherent microglial morphology SPECIES: mouse, C57BL/6 TISSUE/ORGAN: brain, microglial cells TRANSFORMED BY: recombinant retrovirus (v-raf/v-mic) DEPOSITOR: Dr E. Blasi, Dip. The predominant reliance on bromated flame retardants (BFRs) is diminishing with expanded use of alternative organophosphate flame retardants. 普诺赛生物（Procell），您身边的细胞培养专家，顶级细胞供应商，为您提供细胞系、原代细胞、肿瘤细胞、正常细胞、耐药细胞、抗性细胞、细胞株供应、进口细胞株、细胞株进口以及各种人源细胞系等优质产品及服务！请联系400-650-4158，欢迎访问www.procell.com.cn！ Basal glycolytic rate was altered by PBP and TBBPA and compensatory glycolysis by all three.  |  Immunofluorescent confocal microscopy was conducted using an antibody against the GPR18 C-terminus (1:150; green), phalloidin to label actin (1:40; red), and DAPI (1.5 μg/ml) to label the nucleus (blue). Date Updated: 12/18/2017; CRL-1772 cells - coating of flasks It is not necessary to use coated flasks for culture or differentiation of these cells. I need a decent mouse line so will probably buy in the BV-2 but am still looking for a good ... //rdcu.be/6hXK), in which you can also find relevant information on the HMC3 ATCC CRL3304 cells. The cells showed a normal regulation of NO production and a functional response to IFN-gamma, important parameters for appropriate interaction with T cells and neurons. All cell culture must be undertaken in microbiological safety cabinet using aseptic technique to ensure sterility. The ATCC Cell Biology Collection is one of the largest bioresources in the world, and offers a complex array of human, animal, insect, fish and stem cell lines from which to choose. Toxicol Sci. FR exposure did not inhibit response to LPS. So is it necessary to use 0.25% trypsin to digest the cells in subculture? The mouse microglial cell line BV-2 cells, human neuroblastoma cells (SHSY5Y) and mouse hippocampal neuron cell line (HT22) were obtained from ATCC (Rockefeller, USA). Tissue: brain, microglial cells; Transformed by: recombinant retrovirus (v-raf/v-mic). Bioz Stars score: 92/100, based on 3 PubMed citations. *P < 0.05, **P < 0.01, ***P < 0.001 vs. untreated cells. BV-2 cells、 SHSY5Y cells and HT22 cells all grew in complete medium contains 10 % … Figure Legend Snippet: BV-2 microglia and HEK293-GPR18 transfected, but not HEK293 wildtype, cells express GPR18 . BV-2 cells were cultured in A. culbertsoni -conditioned medium for 8 h (Culb 8 h) or for 18 h (Culb 18 h) or in A. astronyxis -conditioned medium for 18 h (Astro 18 h). The E3 Ubiquitin Ligase c-Cbl Inhibits Microglia-Mediated CNS Inflammation by Regulating PI3K/Akt/NF-κB Pathway. Cell culture reagents [Eagle’s Minimum Essential Medium (EMEM, ATCC® 30–2003™) and Fetal Bovine Serum (FBS, ATCC® 30–2020™)] were purchased from ATCC®, and cells were cultured in EMEM supplemented with 10% FBS and antibiotics (100 IU/ml of penicillin and 100 μg/ml of streptomycin, Biochrom AG, Berlin, Germany). The BV-2 cells express the nuclear v-myc and the cytoplasmic v-raf oncogene products as well as the env gp70 antigen at the surface level; The BV-2 cells have morphological, phenotypical … All cell culture must be undertaken in microbiological safety cabinet using aseptic technique to ensure sterility. a , HEK293 wildtype with DAPI and phalloidin. 3,3'-Diindolylmethane inhibits lipopolysaccharide-induced microglial hyperactivation and attenuates brain inflammation. Cell culture and tissue samples Human breast cancer cell lines MCF10A, MCF10F, MCF7, T47D, BT474, BT483, ZR-75-1, MDA-MB361, HBL-100, SKBr3, MDA-MB-468, MDA-MB-453, BT549, MDA-MB-436, MDA-MB-231 as well as the Human Embryonic Kidney cell line HEK293, were purchased from the American Type Culture Collection (ATCC) and grown in accordance with ATCC recommendations. Copyright © 2020 Elsevier B.V. or its licensors or contributors. 2020 Oct 13;10(10):408. doi: 10.3390/metabo10100408. Bioz Stars score: 93/100, based on 5 PubMed citations. They triggered the translocation of NF-kappaB, and a subsequent production of IL-6 in astrocytes. Do not exceed 7 x 10 4 cells/cm 2. doi: 10.1111/j.1471-4159.2008.05633.x Here, we re-evaluated strengths and potential short-comings of BV-2 cells. Abstract. Bioz Stars score: 92/100, based on 12 PubMed citations. These T75 flasks work great for the culturing of BV-2 cells. The role of microglia in neurodegeneration, toxicology and immunity is an expanding area of biomedical research requiring large numbers of animals. Human cell lines, mouse cell lines, cancer cell lines and more are available. These cells grow as a mixture of floating and adherent cells. 93112517) and the adriamycin resistant cell line A2780 ADR (Sigma catalogue no. Chantong B, Kratschmar DV, Nashev LG, Balazs Z, Odermatt A. J Neuroinflammation. Brain Res. Cell culture reagents [Eagle’s Minimum Essential Medium (EMEM, ATCC® 30–2003™) and Fetal Bovine Serum (FBS, ATCC® 30–2020™)] were purchased from ATCC®, and cells were cultured in EMEM supplemented with 10% FBS and antibiotics (100 IU/ml of penicillin and 100 μg/ml of streptomycin, Biochrom AG, Berlin, Germany). 2020 Oct 19;25(20):4796. doi: 10.3390/molecules25204796. 1. Usually BV-2 cells grow every 2 days (and fill up a 75cm2 flask) so you need to subculture. Chiocchio I, Prata C, Mandrone M, Ricciardiello F, Marrazzo P, Tomasi P, Angeloni C, Fiorentini D, Malaguti M, Poli F, Hrelia S. Metabolites. .. All validated cell lines are a result of researchers using the RNAi Consortium (TRC) shRNA library delivered via lentivirus. 107, 557–569. Hydroxysafflor Yellow A Exerts Anti-Inflammatory Effects Mediated by SIRT1 in Lipopolysaccharide-Induced Microglia Activation. USA.gov. Qin X, Chen J, Zhang G, Li C, Zhu J, Xue H, Li J, Guan T, Zheng H, Liu Y, Cai H. Front Pharmacol. Int Immunopharmacol. 2016 Jul 11;13(1):182. doi: 10.1186/s12974-016-0644-1. Basal respiration was altered by PBP; ATP-linked respiration by PBP and TBBPA, and maximum respiration by all three FRs. Das A, Kim SH, Arifuzzaman S, Yoon T, Chai JC, Lee YS, Park KS, Jung KH, Chai YG. BV‐2 cells are not constitu‐tively able to kill tumor cells in vitro, but acquire antitumor activity following an increase in … Epub 2010 Feb 6. 普诺赛生物（Procell），您身边的细胞培养专家，顶级细胞供应商，为您提供细胞系、原代细胞、肿瘤细胞、正常细胞、耐药细胞、抗性细胞、细胞株供应、进口细胞株、细胞株进口以及各种人源细胞系等优质产品及服务！请联系400-650-4158，欢迎访问www.procell.com.cn！ 2019 May;1864(5):704-714. doi: 10.1016/j.bbalip.2019.02.006. The immortalised murine microglial cell line BV-2 has been used frequently as a substitute for PM, but recently doubts were raised as to their suitability. To discuss your PER.C6 Cell Lines demands or to request a proposal, please contact us by The cells were derived from a pleural cavity based lymphomatous effusion. 1. NLRP3 inflammasome activation was assessed using BV-2-ASC (apoptosis-associated speck-like protein containing a CARD) reporter cells to visualize aggregate formation. Due to its popularity, this line has been compared extensively to primary newborn as well as adult microglia. Vascular calcification has severe clinical consequences in a number of diseases, including diabetes, atherosclerosis and end-stage renal disease. Differential migration, LPS-induced cytokine, chemokine, and NO expression in immortalized BV-2 and HAPI cell lines and primary microglial cultures. LPS-induced iNOS expression in Bv-2 cells is suppressed by an oxidative mechanism acting on the JNK pathway--a potential role for neuroprotection. Microglial BV-2 cells are derived from immortalized mouse microglia and exhibit many of the morphological phenotypes and functional characteristics of activated microglia, including phagocytic responses and pro-inflammatory cytokine production . Rat myeloma cell lines: YB2/0, etc. Their response to lipopolysaccharide was compared with the response of microglia in vitro and in vivo. We use cookies to help provide and enhance our service and tailor content and ads. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. Sigma-Aldrich has partnered with The European Collection of Cell Cultures, ECACC, a world leader and recognized expert in the maintenance, cultivation and distribution of authenticated cell lines. Preparing an aseptic environment 1. Data represents total cell count (mean±SEM) as % of controls (n = 4). BV-2 Cell Culture and Treatment.